Genomic variations define divergence of water/wildlife-associated Campylobacter jejuni niche specialists from common clonal complexes

Although the major food-borne pathogen Campylobacter jejuni has been isolated from diverse animal, human and environmental sources, our knowledge of genomic diversity in C. jejuni is based exclusively on human or human food-chain-associated isolates. Studies employing multilocus sequence typing have indicated that some clonal complexes are more commonly associated with particular sources. Using comparative genomic hybridization on a collection of 80 isolates representing diverse sources and clonal complexes, we identified a separate clade comprising a group of water/wildlife isolates of C. jejuni with multilocus sequence types uncharacteristic of human food-chain-associated isolates. By genome sequencing one representative of this diverse group (C. jejuni 1336), and a representative of the bank-vole niche specialist ST-3704 (C. jejuni 414), we identified deletions of genomic regions normally carried by human food-chain-associated C. jejuni. Several of the deleted regions included genes implicated in chicken colonization or in virulence. Novel genomic insertions contributing to the accessory genomes of strains 1336 and 414 were identified. Comparative analysis using PCR assays indicated that novel regions were common but not ubiquitous among the water/wildlife group of isolates, indicating further genomic diversity among this group, whereas all ST-3704 isolates carried the same novel accessory regions. While strain 1336 was able to colonize chicks, strain 414 was not, suggesting that regions specifically absent from the genome of strain 414 may play an important role in this common route of Campylobacter infection of humans. We suggest that the genomic divergence observed constitutes evidence of adaptation leading to niche specialization

Effect of mutual coupling on torque production in switched reluctance motors

In many cases, the normal operation of switched reluctance machines requires excitation of two or more phases simultaneously. When multiple phases are conducting simultaneously, the flux paths from each phase will overlap, which may lead to localized saturation. In such cases, the flux linkage must be considered a function not just of the current in the test winding but of all excited windings. The degree of mutual coupling between phases influences the per-phase magnetization curves and torque characteristics. In machines with even phase numbers, the degree of mutual coupling between phases varies due to discontinuities in the phase polarity arrangement. From nonlinear finite element simulations, it is possible to compare the i - loop diagrams under single-phase and multiphase excitations, and hence the torque produced. The mutual flux linkage from each phase can be calculated separately for each rotor position using the frozen permeability method, to further analyze the mutual coupling effects. For a given excitation current profile, the torque can be maximized by careful arrangement of the phase polarities

Prevalence of Type VI Secretion System in Spanish Campylobacter jejuni Isolates.

Infections from Campylobacter jejuni pose a serious public health problem and are now considered the leading cause of foodborne bacterial gastroenteritis throughout the world. Sequencing of C. jejuni genomes has previously allowed a number of loci to be identified, which encode virulence factors that aid survival and pathogenicity. Recently, a Type VI secretion system (T6SS) consisting of 13 conserved genes was described in C. jejuni strains and recognised to promote pathogenicity and adaptation to the environment. In this study, we determined the presence of this T6SS in 63 Spanish C. jejuni isolates from the food chain and urban effluents using whole-genome sequencing. Our findings demonstrated that nine (14%) strains harboured the 13 ORFs found in prototype strain C. jejuni 108. Further studies will be necessary to determine the prevalence and importance of T6SS-positive C. jejuni strains

The HIV-1 protective-35SNP effect in Caucasians is CD8 T cell mediated

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A 3D forward stratigraphic model of fluvial meander-bend evolution for prediction of point-bar lithofacies architecture

Although fundamental types of fluvial meander-bend transformations – expansion, translation, rotation, and combinations thereof – are widely recognised, the relationship between the migratory behaviour of a meander bend, and its resultant accumulated sedimentary architecture and lithofacies distribution remains relatively poorly understood. Three-dimensional data from both currently active fluvial systems and from ancient preserved successions known from outcrop and subsurface settings are limited. To tackle this problem, a 3D numerical forward stratigraphic model – the Point-Bar Sedimentary Architecture Numerical Deduction (PB-SAND) – has been devised as a tool for the reconstruction and prediction of the complex spatio-temporal migratory evolution of fluvial meanders, their generated bar forms and the associated lithofacies distributions that accumulate as heterogeneous fluvial successions. PB-SAND uses a dominantly geometric modelling approach supplemented by process-based and stochastic model components, and is constrained by quantified sedimentological data derived from modern point bars or ancient successions that represent suitable analogues. The model predicts the internal architecture and geometry of fluvial point-bar elements in three dimensions. The model is applied to predict the sedimentary lithofacies architecture of ancient preserved point-bar and counter-point-bar deposits of the middle Jurassic Scalby Formation (North Yorkshire, UK) to demonstrate the predictive capabilities of PB-SAND in modelling 3D architectures of different types of meander-bend transformations. PB-SAND serves as a practical tool with which to predict heterogeneity in subsurface hydrocarbon reservoirs and water aquifers

Delineation of the Innate and Adaptive T-Cell Immune Outcome in the Human Host in Response to Campylobacter jejuni Infection

Background: Campylobacter jejuni is the most prevalent cause of bacterial gastroenteritis worldwide. Despite the significant health burden this infection presents, molecular understanding of C. jejuni-mediated disease pathogenesis remains poorly defined. Here, we report the characterisation of the early, innate immune response to C. jejuni using an ex-vivo human gut model of infection. Secondly, impact of bacterial-driven dendritic cell activation on T-cell mediated immunity was also sought.Methodology: Healthy, control paediatric terminal ileum or colonic biopsy tissue was infected with C. jejuni for 8-12 hours. Bacterial colonisation was followed by confocal microscopy and mucosal innate immune responses measured by ELISA. Marked induction of IFN gamma with modest increase in IL-22 and IL-17A was noted. Increased mucosal IL-12, IL-23, IL-1 beta and IL-6 were indicative of a cytokine milieu that may modulate subsequent T-cell mediated immunity. C. jejuni-driven human monocyte-derived dendritic cell activation was followed by analyses of T cell immune responses utilising flow cytometry and ELISA. Significant increase in Th-17, Th-1 and Th-17/Th-1 double-positive cells and corresponding cytokines was observed. The ability of IFN gamma, IL-22 and IL-17 cytokines to exert host defence via modulation of C. jejuni adhesion and invasion to intestinal epithelia was measured by standard gentamicin protection assay.Conclusions: Both innate and adaptive T cell-immunity to C. jejuni infection led to the release of IFN gamma, IL-22 and IL-17A; suggesting a critical role for this cytokine triad in establishing host anti-microbial immunity during the acute and effectors phase of infection. In addition, to their known anti-microbial functions; IL-17A and IL-17F reduced the number of intracellular C. jejuni in intestinal epithelia, highlighting a novel aspect of how IL-17 family members may contribute to protective immunity against C. jejuni

Effects of manufacturing tolerances of permanent magnets in fractional slot permanent magnet synchronous machines

The fractional slot permanent magnet synchronous machines are well-known for their low torque ripple. However, in mass-production machines, not all the permanent magnets are identical due to the manufacturing tolerances and new torque harmonics could appear. In addition, the magnetic field is modified and unbalanced magnetic pull (UMP) could happen. Apart from that it is important to study the effect on vibrations since the magnetic field is no longer ideal. So, here, a study of the effect of unevenly magnetised permanent magnet distribution on torque ripples, unbalance magnetic pull, and vibrations is proposed. Apart from that, the tolerance grade sensitivity is studied. Finally, experimental tests show good agreement with finite element analysis